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proteinlso gst resin  (TransGen biotech co)

 
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    Structured Review

    TransGen biotech co proteinlso gst resin
    a TaSG-D1/TaSG-D1 E286K interact with the C-terminus of TaPIF4 in a Y2H assay. 35 mM 3-amino-1,2,4-triazole (3-AT) was used to suppress self-activation in the TaSG-D1 E286K and TaPIF4 interaction assay. SD-L/W stands for media lacking Leucine and Tryptophan. SD-L/W/H/A stands for media lacking Leucine, Tryptophan, Histidine and Adenine. b TaSG-D1/TaSG-D1 E286K interact with TaPIF4 in N. benthamiana leaves in an LCI assay. The experiment was performed five times. c TaSG-D1/TaSG-D1 E286K interact with TaPIF4 in vitro in a pull-down assay. <t>GST/GST-TaPIF4</t> were incubated with His-TaSG-D1/His-TaSG-D1 E286K . Protein mixture was immunoprecipitated with <t>Proteinlso</t> GST Resin and detected with anti-His (upper panel) and anti-GST (lower panel) antibodies. The arrow in lower panel (~75 KDa) indicates GST-TaPIF4. “**” in the upper panel indicates non-specific band, the arrow in the upper panel indicates non-phosphorylated His-TaSG-D1/His-TaSG-D1 E286K (~48 KDa), “*” in the upper panel indicates self-phosphorylated His-TaSG-D1/His-TaSG-D1 E286K . Three independent experiments were performed. d TaSG-D1/TaSG-D1 E286K interact with TaPIF4 in vivo in a Co-IP assay. 35 S: TaSG-D1-GFP/35 S: TaSG-D1 E286K -GFP/35 S: GFP and 35 S: TaPIF4-Myc were co-expressed in N. benthamiana leaves. Protein extract was immunoprecipitated with anti-GFP magnetic beads and detected using anti-GFP (lower panel) and anti-Myc (upper panel) antibodies. Three independent experiments were performed. Source data are provided as a file.
    Proteinlso Gst Resin, supplied by TransGen biotech co, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/proteinlso+gst+resin/pmc10920922-222-12-15?v=TransGen+biotech+co
    Average 90 stars, based on 1 article reviews
    proteinlso gst resin - by Bioz Stars, 2026-08
    90/100 stars

    Images

    1) Product Images from "Natural variation of STKc_GSK3 kinase TaSG-D1 contributes to heat stress tolerance in Indian dwarf wheat"

    Article Title: Natural variation of STKc_GSK3 kinase TaSG-D1 contributes to heat stress tolerance in Indian dwarf wheat

    Journal: Nature Communications

    doi: 10.1038/s41467-024-46419-0

    a TaSG-D1/TaSG-D1 E286K interact with the C-terminus of TaPIF4 in a Y2H assay. 35 mM 3-amino-1,2,4-triazole (3-AT) was used to suppress self-activation in the TaSG-D1 E286K and TaPIF4 interaction assay. SD-L/W stands for media lacking Leucine and Tryptophan. SD-L/W/H/A stands for media lacking Leucine, Tryptophan, Histidine and Adenine. b TaSG-D1/TaSG-D1 E286K interact with TaPIF4 in N. benthamiana leaves in an LCI assay. The experiment was performed five times. c TaSG-D1/TaSG-D1 E286K interact with TaPIF4 in vitro in a pull-down assay. GST/GST-TaPIF4 were incubated with His-TaSG-D1/His-TaSG-D1 E286K . Protein mixture was immunoprecipitated with Proteinlso GST Resin and detected with anti-His (upper panel) and anti-GST (lower panel) antibodies. The arrow in lower panel (~75 KDa) indicates GST-TaPIF4. “**” in the upper panel indicates non-specific band, the arrow in the upper panel indicates non-phosphorylated His-TaSG-D1/His-TaSG-D1 E286K (~48 KDa), “*” in the upper panel indicates self-phosphorylated His-TaSG-D1/His-TaSG-D1 E286K . Three independent experiments were performed. d TaSG-D1/TaSG-D1 E286K interact with TaPIF4 in vivo in a Co-IP assay. 35 S: TaSG-D1-GFP/35 S: TaSG-D1 E286K -GFP/35 S: GFP and 35 S: TaPIF4-Myc were co-expressed in N. benthamiana leaves. Protein extract was immunoprecipitated with anti-GFP magnetic beads and detected using anti-GFP (lower panel) and anti-Myc (upper panel) antibodies. Three independent experiments were performed. Source data are provided as a file.
    Figure Legend Snippet: a TaSG-D1/TaSG-D1 E286K interact with the C-terminus of TaPIF4 in a Y2H assay. 35 mM 3-amino-1,2,4-triazole (3-AT) was used to suppress self-activation in the TaSG-D1 E286K and TaPIF4 interaction assay. SD-L/W stands for media lacking Leucine and Tryptophan. SD-L/W/H/A stands for media lacking Leucine, Tryptophan, Histidine and Adenine. b TaSG-D1/TaSG-D1 E286K interact with TaPIF4 in N. benthamiana leaves in an LCI assay. The experiment was performed five times. c TaSG-D1/TaSG-D1 E286K interact with TaPIF4 in vitro in a pull-down assay. GST/GST-TaPIF4 were incubated with His-TaSG-D1/His-TaSG-D1 E286K . Protein mixture was immunoprecipitated with Proteinlso GST Resin and detected with anti-His (upper panel) and anti-GST (lower panel) antibodies. The arrow in lower panel (~75 KDa) indicates GST-TaPIF4. “**” in the upper panel indicates non-specific band, the arrow in the upper panel indicates non-phosphorylated His-TaSG-D1/His-TaSG-D1 E286K (~48 KDa), “*” in the upper panel indicates self-phosphorylated His-TaSG-D1/His-TaSG-D1 E286K . Three independent experiments were performed. d TaSG-D1/TaSG-D1 E286K interact with TaPIF4 in vivo in a Co-IP assay. 35 S: TaSG-D1-GFP/35 S: TaSG-D1 E286K -GFP/35 S: GFP and 35 S: TaPIF4-Myc were co-expressed in N. benthamiana leaves. Protein extract was immunoprecipitated with anti-GFP magnetic beads and detected using anti-GFP (lower panel) and anti-Myc (upper panel) antibodies. Three independent experiments were performed. Source data are provided as a file.

    Techniques Used: Y2H Assay, Activation Assay, In Vitro, Pull Down Assay, Incubation, Immunoprecipitation, In Vivo, Co-Immunoprecipitation Assay, Magnetic Beads



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    proteinlso gst resin  (TransGen biotech co)
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    TransGen biotech co proteinlso gst resin
    a TaSG-D1/TaSG-D1 E286K interact with the C-terminus of TaPIF4 in a Y2H assay. 35 mM 3-amino-1,2,4-triazole (3-AT) was used to suppress self-activation in the TaSG-D1 E286K and TaPIF4 interaction assay. SD-L/W stands for media lacking Leucine and Tryptophan. SD-L/W/H/A stands for media lacking Leucine, Tryptophan, Histidine and Adenine. b TaSG-D1/TaSG-D1 E286K interact with TaPIF4 in N. benthamiana leaves in an LCI assay. The experiment was performed five times. c TaSG-D1/TaSG-D1 E286K interact with TaPIF4 in vitro in a pull-down assay. <t>GST/GST-TaPIF4</t> were incubated with His-TaSG-D1/His-TaSG-D1 E286K . Protein mixture was immunoprecipitated with <t>Proteinlso</t> GST Resin and detected with anti-His (upper panel) and anti-GST (lower panel) antibodies. The arrow in lower panel (~75 KDa) indicates GST-TaPIF4. “**” in the upper panel indicates non-specific band, the arrow in the upper panel indicates non-phosphorylated His-TaSG-D1/His-TaSG-D1 E286K (~48 KDa), “*” in the upper panel indicates self-phosphorylated His-TaSG-D1/His-TaSG-D1 E286K . Three independent experiments were performed. d TaSG-D1/TaSG-D1 E286K interact with TaPIF4 in vivo in a Co-IP assay. 35 S: TaSG-D1-GFP/35 S: TaSG-D1 E286K -GFP/35 S: GFP and 35 S: TaPIF4-Myc were co-expressed in N. benthamiana leaves. Protein extract was immunoprecipitated with anti-GFP magnetic beads and detected using anti-GFP (lower panel) and anti-Myc (upper panel) antibodies. Three independent experiments were performed. Source data are provided as a file.
    Proteinlso Gst Resin, supplied by TransGen biotech co, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/proteinlso+gst+resin/pmc10920922-222-12-15?v=TransGen+biotech+co
    Average 90 stars, based on 1 article reviews
    proteinlso gst resin - by Bioz Stars, 2026-08
    90/100 stars
    		  Buy from Supplier
    proteinlso® gst resin  (TransGen biotech co)
    90
    TransGen biotech co proteinlso® gst resin
    a TaSG-D1/TaSG-D1 E286K interact with the C-terminus of TaPIF4 in a Y2H assay. 35 mM 3-amino-1,2,4-triazole (3-AT) was used to suppress self-activation in the TaSG-D1 E286K and TaPIF4 interaction assay. SD-L/W stands for media lacking Leucine and Tryptophan. SD-L/W/H/A stands for media lacking Leucine, Tryptophan, Histidine and Adenine. b TaSG-D1/TaSG-D1 E286K interact with TaPIF4 in N. benthamiana leaves in an LCI assay. The experiment was performed five times. c TaSG-D1/TaSG-D1 E286K interact with TaPIF4 in vitro in a pull-down assay. <t>GST/GST-TaPIF4</t> were incubated with His-TaSG-D1/His-TaSG-D1 E286K . Protein mixture was immunoprecipitated with <t>Proteinlso</t> GST Resin and detected with anti-His (upper panel) and anti-GST (lower panel) antibodies. The arrow in lower panel (~75 KDa) indicates GST-TaPIF4. “**” in the upper panel indicates non-specific band, the arrow in the upper panel indicates non-phosphorylated His-TaSG-D1/His-TaSG-D1 E286K (~48 KDa), “*” in the upper panel indicates self-phosphorylated His-TaSG-D1/His-TaSG-D1 E286K . Three independent experiments were performed. d TaSG-D1/TaSG-D1 E286K interact with TaPIF4 in vivo in a Co-IP assay. 35 S: TaSG-D1-GFP/35 S: TaSG-D1 E286K -GFP/35 S: GFP and 35 S: TaPIF4-Myc were co-expressed in N. benthamiana leaves. Protein extract was immunoprecipitated with anti-GFP magnetic beads and detected using anti-GFP (lower panel) and anti-Myc (upper panel) antibodies. Three independent experiments were performed. Source data are provided as a file.
    Proteinlso® Gst Resin, supplied by TransGen biotech co, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/proteinlso+gst+resin/10__1017_slash_s0007485323000184-52-10-13?v=TransGen+biotech+co
    Average 90 stars, based on 1 article reviews
    proteinlso® gst resin - by Bioz Stars, 2026-08
    90/100 stars
    		  Buy from Supplier

    Image Search Results


    a TaSG-D1/TaSG-D1 E286K interact with the C-terminus of TaPIF4 in a Y2H assay. 35 mM 3-amino-1,2,4-triazole (3-AT) was used to suppress self-activation in the TaSG-D1 E286K and TaPIF4 interaction assay. SD-L/W stands for media lacking Leucine and Tryptophan. SD-L/W/H/A stands for media lacking Leucine, Tryptophan, Histidine and Adenine. b TaSG-D1/TaSG-D1 E286K interact with TaPIF4 in N. benthamiana leaves in an LCI assay. The experiment was performed five times. c TaSG-D1/TaSG-D1 E286K interact with TaPIF4 in vitro in a pull-down assay. GST/GST-TaPIF4 were incubated with His-TaSG-D1/His-TaSG-D1 E286K . Protein mixture was immunoprecipitated with Proteinlso GST Resin and detected with anti-His (upper panel) and anti-GST (lower panel) antibodies. The arrow in lower panel (~75 KDa) indicates GST-TaPIF4. “**” in the upper panel indicates non-specific band, the arrow in the upper panel indicates non-phosphorylated His-TaSG-D1/His-TaSG-D1 E286K (~48 KDa), “*” in the upper panel indicates self-phosphorylated His-TaSG-D1/His-TaSG-D1 E286K . Three independent experiments were performed. d TaSG-D1/TaSG-D1 E286K interact with TaPIF4 in vivo in a Co-IP assay. 35 S: TaSG-D1-GFP/35 S: TaSG-D1 E286K -GFP/35 S: GFP and 35 S: TaPIF4-Myc were co-expressed in N. benthamiana leaves. Protein extract was immunoprecipitated with anti-GFP magnetic beads and detected using anti-GFP (lower panel) and anti-Myc (upper panel) antibodies. Three independent experiments were performed. Source data are provided as a file.

    Journal: Nature Communications

    Article Title: Natural variation of STKc_GSK3 kinase TaSG-D1 contributes to heat stress tolerance in Indian dwarf wheat

    doi: 10.1038/s41467-024-46419-0

    Figure Lengend Snippet: a TaSG-D1/TaSG-D1 E286K interact with the C-terminus of TaPIF4 in a Y2H assay. 35 mM 3-amino-1,2,4-triazole (3-AT) was used to suppress self-activation in the TaSG-D1 E286K and TaPIF4 interaction assay. SD-L/W stands for media lacking Leucine and Tryptophan. SD-L/W/H/A stands for media lacking Leucine, Tryptophan, Histidine and Adenine. b TaSG-D1/TaSG-D1 E286K interact with TaPIF4 in N. benthamiana leaves in an LCI assay. The experiment was performed five times. c TaSG-D1/TaSG-D1 E286K interact with TaPIF4 in vitro in a pull-down assay. GST/GST-TaPIF4 were incubated with His-TaSG-D1/His-TaSG-D1 E286K . Protein mixture was immunoprecipitated with Proteinlso GST Resin and detected with anti-His (upper panel) and anti-GST (lower panel) antibodies. The arrow in lower panel (~75 KDa) indicates GST-TaPIF4. “**” in the upper panel indicates non-specific band, the arrow in the upper panel indicates non-phosphorylated His-TaSG-D1/His-TaSG-D1 E286K (~48 KDa), “*” in the upper panel indicates self-phosphorylated His-TaSG-D1/His-TaSG-D1 E286K . Three independent experiments were performed. d TaSG-D1/TaSG-D1 E286K interact with TaPIF4 in vivo in a Co-IP assay. 35 S: TaSG-D1-GFP/35 S: TaSG-D1 E286K -GFP/35 S: GFP and 35 S: TaPIF4-Myc were co-expressed in N. benthamiana leaves. Protein extract was immunoprecipitated with anti-GFP magnetic beads and detected using anti-GFP (lower panel) and anti-Myc (upper panel) antibodies. Three independent experiments were performed. Source data are provided as a file.

    Article Snippet: For pull-down assays of interaction, 5 µg GST or GST-TaPIF4 coupled with Proteinlso GST Resin (TransGen Biotech, DP201-01) were incubated with 5 µg His-TaSG-D1 (pET-28a)/5 µg His-TaSG-D1 E286K (pET-28a) at 4 °C for 2 h with gentle mixing.

    Techniques: Y2H Assay, Activation Assay, In Vitro, Pull Down Assay, Incubation, Immunoprecipitation, In Vivo, Co-Immunoprecipitation Assay, Magnetic Beads